{"id":1488,"date":"2022-02-27T17:37:44","date_gmt":"2022-02-27T16:37:44","guid":{"rendered":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/?p=1488"},"modified":"2025-11-09T15:47:59","modified_gmt":"2025-11-09T14:47:59","slug":"spectrophotometrie","status":"publish","type":"post","link":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/spectrophotometrie\/","title":{"rendered":"Un exemple d&rsquo;animation pour travailler la spectrophotom\u00e9trie"},"content":{"rendered":"\n<p class=\"has-drop-cap has-text-color\" style=\"color:#001b2b;font-size:28px;font-style:normal;font-weight:800;letter-spacing:3px\">L&rsquo;universit\u00e9 du Colorado a mis au point une s\u00e9rie d&rsquo;animations pour simuler diverses exp\u00e9rimentations avec nos \u00e9l\u00e8ves.<\/p>\n\n\n\n<h2 class=\"wp-block-heading has-text-color has-medium-font-size\" style=\"color:#016ba9\"><strong>Pour nos s\u00e9ries technologiques, l&rsquo;une d&rsquo;elle est particuli\u00e8rement int\u00e9ressante car portant sur la spectrophotom\u00e9trie (source: <a rel=\"noreferrer noopener\" href=\"https:\/\/phet.colorado.edu\/sims\/html\/beers-law-lab\/latest\/beers-law-lab_en.html\" target=\"_blank\">https:\/\/phet.colorado.edu\/sims\/html\/beers-law-lab\/latest\/beers-law-lab_en.html<\/a>):<\/strong><\/h2>\n\n\n\n<ul style=\"color:#026caa\" class=\"wp-block-list has-text-color has-medium-font-size\">\n<li><strong>Cliquer sur \u00ab\u00a0Loi de Beer-Lambert\u00a0\u00bb<\/strong><\/li>\n\n\n\n<li><strong>Vous pouvez ensuite: mettre en route le spectrophotom\u00e9tre (bouton rouge),  modifier la longueur de la cuve, faire varier la longueur d&rsquo;onde, la concentration&#8230;<\/strong><\/li>\n<\/ul>\n\n\n<style>\n       .errordiv { padding:10px; margin:10px; border: 1px solid #555555;color: #000000;background-color: #f8f8f8; width:500px; }#advanced_iframe {visibility:visible;opacity:1;vertical-align:top;}.ai-info-bottom-iframe { position: fixed; z-index: 10000; bottom:0; left: 0; margin: 0px; text-align: center; width: 100%; background-color: #ff9999; padding-left: 5px;padding-bottom: 5px; border-top: 1px solid #aaa } a.ai-bold {font-weight: bold;}#ai-layer-div-advanced_iframe p {height:100%;margin:0;padding:0}<\/style><script type=\"text\/javascript\">var ai_iframe_width_advanced_iframe = 0;var ai_iframe_height_advanced_iframe = 0;var aiOnloadScrollTop=\"true\";var aiShowDebug=false;\n\t\tif (typeof aiReadyCallbacks === 'undefined') {\n\t\t\tvar aiReadyCallbacks = [];\n\t\t} else if (!(aiReadyCallbacks instanceof Array)) {\n\t\t\tvar aiReadyCallbacks = [];\n\t\t}    function aiShowIframeId(id_iframe) { jQuery(\"#\"+id_iframe).css(\"visibility\", \"visible\");    }    function aiResizeIframeHeight(height) { aiResizeIframeHeight(height,advanced_iframe); }    function aiResizeIframeHeightId(height,width,id) {aiResizeIframeHeightById(id,height);}<\/script><a href=\"https:\/\/phet.colorado.edu\/sims\/html\/beers-law-lab\/latest\/beers-law-lab_fr.html\" target=\"_blank\" rel=\"noopener\">Contenu embarqu\u00e9 non autoris\u00e9<\/a><script type=\"text\/javascript\">var ifrm_advanced_iframe = document.getElementById(\"advanced_iframe\");var hiddenTabsDoneadvanced_iframe = false;\nfunction resizeCallbackadvanced_iframe() {}<\/script><script type=\"text\/javascript\"><\/script><p style=\"display:block !important; visibility:visible !important;margin: -18px 14px 0 0;padding-left: 3px;padding-top:3px;background: white; overflow: hidden; position: relative; line-height:15px;width: fit-content;\"><small style=\"display:block !important;visibility:visible !important\">powered by Advanced iFrame<\/small><\/p>\n\n\n<h2 class=\"wp-block-heading has-text-color has-medium-font-size\" style=\"color:#006caa\">   <strong>Le code source a \u00e9t\u00e9 publi\u00e9 (<a rel=\"noreferrer noopener\" href=\"https:\/\/github.com\/phetsims\/beers-law-lab\" target=\"_blank\">https:\/\/github.com\/phetsims\/beers-law-lab<\/a>), les traductions sont modifiables (connaissances de base en HTML5 et javascript requises) et l&rsquo;outil peut \u00e9galement \u00eatre modifi\u00e9 pour d&rsquo;autres applications. Un exemple vous est donn\u00e9 ci-dessous pour travailler sur le dosage de l&rsquo;ADN<\/strong> <strong>(cliquer sur la partie modifi\u00e9e: Dosage de l&rsquo;ADN par spectrophotom\u00e9trie)<\/strong>.<\/h2>\n\n\n\n<ul style=\"color:#005383\" class=\"wp-block-list has-text-color has-medium-font-size\">\n<li><strong>Cliquer sur \u00ab\u00a0Dosage de l&rsquo;ADN par spectrophotom\u00e9trie<\/strong><\/li>\n<\/ul>\n\n\n\n<ul style=\"color:#005c92\" class=\"wp-block-list has-text-color has-medium-font-size\">\n<li><strong>Cet outil permet aux \u00e9l\u00e8ves de d\u00e9terminer \u00e0 quelle longueur d&rsquo;onde le dosage de l&rsquo;ADN sera r\u00e9alis\u00e9<\/strong><\/li>\n<\/ul>\n\n\n<style>\n       .errordiv { padding:10px; margin:10px; border: 1px solid #555555;color: #000000;background-color: #f8f8f8; width:500px; }#advanced_iframe_2 {visibility:visible;opacity:1;vertical-align:top;}.ai-info-bottom-iframe { position: fixed; z-index: 10000; bottom:0; left: 0; margin: 0px; text-align: center; width: 100%; background-color: #ff9999; padding-left: 5px;padding-bottom: 5px; border-top: 1px solid #aaa } a.ai-bold {font-weight: bold;}#ai-layer-div-advanced_iframe_2 p {height:100%;margin:0;padding:0}<\/style><script type=\"text\/javascript\">var ai_iframe_width_advanced_iframe_2 = 0;var ai_iframe_height_advanced_iframe_2 = 0;var aiOnloadScrollTop=\"true\";var aiShowDebug=false;\n\t\tif (typeof aiReadyCallbacks === 'undefined') {\n\t\t\tvar aiReadyCallbacks = [];\n\t\t} else if (!(aiReadyCallbacks instanceof Array)) {\n\t\t\tvar aiReadyCallbacks = [];\n\t\t}    function aiShowIframeId(id_iframe) { jQuery(\"#\"+id_iframe).css(\"visibility\", \"visible\");    }    function aiResizeIframeHeight(height) { aiResizeIframeHeight(height,advanced_iframe_2); }    function aiResizeIframeHeightId(height,width,id) {aiResizeIframeHeightById(id,height);}<\/script><a href=\"https:\/\/biotechnologies-genie-biologique-medias.discipline.ac-lille.fr\/dosage_ADN.html\" target=\"_blank\" rel=\"noopener\">Contenu embarqu\u00e9 non autoris\u00e9<\/a><script type=\"text\/javascript\">var ifrm_advanced_iframe_2 = document.getElementById(\"advanced_iframe_2\");var hiddenTabsDoneadvanced_iframe_2 = false;\nfunction resizeCallbackadvanced_iframe_2() {}<\/script><script type=\"text\/javascript\"><\/script><p style=\"display:block !important; visibility:visible !important;margin: -18px 14px 0 0;padding-left: 3px;padding-top:3px;background: white; overflow: hidden; position: relative; line-height:15px;width: fit-content;\"><small style=\"display:block !important;visibility:visible !important\">powered by Advanced iFrame<\/small><\/p>\n\n\n<h2 class=\"wp-block-heading has-text-color has-medium-font-size\" style=\"color:#006aa7\">  <strong>Si vous avez d&rsquo;autres id\u00e9es de modifications n&rsquo;h\u00e9sitez pas \u00e0 nous contacter via la rubrique \u00ab\u00a0CONTACT\u00a0\u00bb ou en laissant un commentaire ci-dessous.<\/strong><\/h2>\n","protected":false},"excerpt":{"rendered":"<p>L&rsquo;universit\u00e9 du Colorado a mis au point une s\u00e9rie d&rsquo;animations pour simuler diverses exp\u00e9rimentations avec nos \u00e9l\u00e8ves. Pour nos s\u00e9ries technologiques, l&rsquo;une d&rsquo;elle est particuli\u00e8rement int\u00e9ressante car portant sur la [&hellip;]<\/p>\n","protected":false},"author":68,"featured_media":1660,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ngg_post_thumbnail":0,"footnotes":""},"categories":[1],"tags":[],"class_list":{"0":"post-1488","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-articles","8":"czr-hentry"},"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-12 19:05:04","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/posts\/1488","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/users\/68"}],"replies":[{"embeddable":true,"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/comments?post=1488"}],"version-history":[{"count":26,"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/posts\/1488\/revisions"}],"predecessor-version":[{"id":3794,"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/posts\/1488\/revisions\/3794"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/media\/1660"}],"wp:attachment":[{"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/media?parent=1488"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/categories?post=1488"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pedagogie.ac-lille.fr\/biotechnologies-genie-biologique\/wp-json\/wp\/v2\/tags?post=1488"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}